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Most Drosera (Sundews) will do well in the same media mix.

Most Sundews will work with the same tissue culture techniques & media, even tuberous. I have had success with the following Sundew's using the same media. 99.5% of Sundews will do fine in 20 - 50% MS, as will all carnivorous plants.

Sundews tissue culture fine without hormones. Hormones are used to create a lot of plants.

Tuberous Sundew Seeds generally need a hot stratification period or soaked in GA3. Temperatures are better at 62 - 72° and photoperiods of 10 - 12 hrs. Start increasing the photoperiod after they germinate, you want a 16 hr photoperiod by the fourth month to help induce tuberizaton. Tuberous sundews are hard to get out of tc any other way


If you use hormones transfer to plain or rooting media after 6 - 12 weeks. You want good leaf formation before placing in rooting media, transfer to plain media for 2 - 8 weeks first if need be, they generally root during this time anyway. After the initial culture further division can be started at any time.

Drosera Adelae Tissue Culture

Difficulty:      Moderate
Usable Parts:      Leaves, Roots, Seeds, Flower Stalk

Sterilization:

Seeds:
15 min. 10% bleach | 2 - 5 min. rinse
or
Soak 4 hrs in 1-50 PPM (2%)

Leaves/Flower Stalk:
Alcohol dip | 10 to 15 min 10% bleach | 2 - 5 min rinse

Roots:
Rinse in running water | 10 min 10% bleach | 2 - 5 min rinse

PPM Calculator <-- Takes you to the sterilization page where the PPM Calculator is, easily calculate PPM %

Tissue Culture Gathering Techniques:

Leaves
Cut the leaves off as close to the stem as possible without cutting into the actual stem itself. Taking new growth could harm the plant (so don't) but you want to get good looking healthy leaves.

Roots
For plants that have long thick roots you can generally take half of a root or two without harming the plant to much. Simply cut half a root off with a razor blade or sharp scissors. Or you can use root tips. Soak mother plant in SuperThrive before re-potting. You can cut the roots into one inch segments but I usually wait until after the sterilization process, per kit manual.

Flower Stalk
You can cut the flower stalk free from the plant at anytime, even after seed set. Cut it as low as you can without harming the plant. Cut into 2" pieces. Treat the same as a leaf cutting.

Media:
4 cups water | 1/2 teaspoon MS w/Vitamins (50% MS) | 2 tablespoons sugar (25g) | 1.5 ml PPM | 2 ml BAP (1mg/ml) | PH 5.6

For seeds leave out BAP.
Seeds can be sterilized by soaking in 1-50 PPM (2%) for 4 hrs. Best results will be achieved by making 2% PPM with sterile media before adding sucrose or adjusting PH.

Cape Sundew Tissue Culture

Difficulty:      Easy
Usable Parts:      Leaves, Roots, Seeds, Flower Stalk

Sterilization:

Seeds:
8 min. 10% bleach | 2 - 5 min. rinse
or
4 hrs 1-50 PPM (2%)

Leaves/Flower Stalk:
Alchohol dip | 20 min 10% bleach | 10 min rinse

Roots:
Rinse in bottled or RO water before starting.
8 min 10% bleach | 5 min rinse | 1 hr 1-50 PPM (2%) | No Rinse

Tissue Culture Gathering Technique:

Flower Stalks:
You can cut the flower stalk free from the plant at anytime, even after seed set. Cut it as low as you can without harming the plant. Cut into 2" pieces. Treat the same as a leaf cutting.

Leaves:
Cut the leaves off pretty close to the stem. You want to get good looking dewy leaves. After sterilization cut the end of the stem off if it turns white from the bleach.

Roots:
You can generally take half of a root without harming the plant to much as long as it has several healthy roots. Simply cut half a root off with a razor blade or sharp scissors. You can cut roots into one inch segments but I usually wait until after the sterilization process, per kit manual.

Media:
4 cups water | 1/2 teaspoon MS w/Vitamins (50% MS) | 2 tablespoons sugar (25g)| 1.5 ml PPM | 2 ml BAP (1mg/ml) | PH 5.6
For seeds leave out BAP.
Seeds can be sterilized by soaking in 1-50 PPM (2%) for 4 hrs. Best results will be achieved by making 2% PPM with sterile media before adding sucrose or adjusting PH.

Drosera Capillaris (long arm)Tissue Culture

Difficulty:      Easy
Usable Parts:      Leaves, Seeds, Flower Stalk

Sterilization:

Seeds:
8 min. 10% bleach | 2 - 5 min. rinse
or
4 hrs 1-50 PPM (2%)

Leaves/Flower Stalks:
Alchohol dip | 1 min peroxide | 10 min 10% bleach | 10 min rinse

Tissue Culture Gathering Technique:

Leaves
Cut the leaves off pretty close to the center of the roset. Taking new growth could harm the plant. After sterilization cut the end of the stem off if it turns white from the bleach. Dewey leaves do not seem to be needed with these. Any pad that can be sterilized works fine.

Flower Stalk
You can cut the flower stalk free from the plant at anytime, even after seed set. Cut it as low as you can without harming the plant. Cut into smaller pieces if needed. Treat the same as a leaf cutting.

Roots
I do not recommend using the roots as these have very small fine roots. You can try the root tips buy it can harm the plant.

Media:
4 cups water | 1/2 teaspoon MS w/Vitamins (50% MS) | 2 tablespoons sugar (25g) | 1.5 ml PPM | 2 ml BAP (1mg/ml) | PH 5.6
For seeds leave out BAP.
Seeds can be sterilized by soaking in 1-50 PPM (2%) for 4 hrs, as can flower stalks. Best results will be achieved by making 2% PPM with sterile media before adding sucrose or adjusting PH.

The above tissue culture media/techniques should work for almost all Drosera. Generally 25 - 50% MS does fine.

If you fail with a species more than twice substitute BAP with 2iP.

Should you need to induce rooting substitute BAP with 2 mL NAA (1mg/ml). Drosera generally need no help rooting.


D. filiformis tissue culture
1

filiformis tissue culture 2
2
kitchen tissue culture 3
3
tissue culture shoot growth
4
kitchen tissue culture 5
5
seed tissue culture
D. Petiolaris seed culture
1 D. Filiformis leaf app. 1" w/dormant bud.
2 It grew a single leaf from the bud about 2" long. Several plantlets then sprouted from the bud.
3 The bud was separated from the new leaf and cultured in plain media to root.
4 The leaf that grew was re-cultured to induce more shoot growth.
5 Now there are 3 jars that look like this one, all from that little bud.

The stem and leaf tip are swelling, getting ready to produce plantlets.





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